逆向电刺激大鼠单侧脊神经背根产生远距离抗炎效应

摘要

目的观察逆向电刺激(ADES)大鼠单侧脊神经背根对不同部位的远距离抗炎效应。方法选用大鼠背部皮神经交互兴奋和足底注射2%福尔马林(FM)为2个不同部位的炎症模型。以皮神经交互兴奋后神经纤维的放电频率及机械阈值变化和FM诱发脊髓背角磷酸化cAMP应答原件结合蛋白(p-CREB)表达增高为观测指标。分别观察ADES单侧脊神经背根，对上述指标的影响。结果ADES单侧脊神经背根可以使背部脊神经皮支C类和A啄纤维交互兴奋受到抑制，并抑制对侧足底FM诱发的脊髓p-CREB的表达增高。结论ADES大鼠脊神经背根具有远距离抗炎效应。%Objective To observe the anti-inflammation effect of antidromic electronic stimulation of rat dorsal roots. Methods Cross-excitation between adjacent primary afferent fibers and subcutaneous injection of 2% FM were adopted here as two inflammatory models. The firing rate and mechanical threshold of single units after cross-excitation were collected. The phosphorylated cAMP response element binding protein (p-CREB) level in spinal cord induced by formalin was visualized with immunohistology. Results Antidromic electronic stimulation (ADES) of dorsal roots could significantly inhibit the p-CREB over expression to FM injected in the contralateral hindpaw, and the increase in firing rate and mechanical threshold induced by cross-excitation were also inhibited by the ADES. Conclusion Antidromic electronic stimulation of the dorsal root may produce a systemic anti-inflamma-tion capacity.