目的 探讨短时受精的临床应用价值.方法 全部病例接受常规超促排卵治疗获得卵母细胞,根据患者接受体外受精(IVF)的不同方式分为2组:短时受精组(n＝35),采用精卵4孔皿混合培养,加精后4 h将卵母细胞转移至分裂期胚胎培养液,共35个周期;传统受精组(n＝35),采用传统的IVF方式,加精后18 h拆除卵母细胞外围颗粒,随后将卵母细胞转移至分裂期胚胎培养液行早期胚胎体外培养,共35个周期.分别比较两组MⅡ率、受精率、正常受精率、总卵裂率、双原核(2PN)卵裂率及妊娠率,并进行统计分析.结果 短时受精组共获卵母细胞数629枚,MⅡ数577枚,受精数560枚,正常受精数500枚,总卵裂数549枚,2PN卵裂数500枚,临床妊娠18例;传统受精组共获卵母细胞594枚,M Ⅱ数550枚,受精数523枚,正常受精数463枚,总卵裂数495枚,2PN卵裂数457枚,临床妊娠12例.两组女方年龄差异无统计学意义,受精率、正常受精率、总卵裂率及2PN卵裂率的组间比较差异无统计学意义,妊娠率的组间比较差异有统计学意义(P0. 05 ), while the pregnancy rate in the testing group( 51. 43% ) was significant higher than that( 34. 29% ) in the control group, as well as there was a significant difference in that between both the groups ( P < 0. 05 ). Conclusion In comparison to convention IVF, the clinical effect resulting from short-time IVF is much better, and short-time IVF can replace convention IVF as a modified IVF programme.
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