CD44 v6和FOXD3在人胃癌组织中的表达及临床意义

摘要

目的 研究CD44v6和FOXD3蛋白在人胃癌组织中的表达及其与胃癌生物学行为的关系. 方法 采用免疫组化Envision二步法检测两种蛋白在100例人胃癌组织及40例癌旁正常胃黏膜组织和110例人胃癌组织芯片中的表达.结果 传统病理切片中CD44v6 蛋白在胃癌组织和癌旁正常胃黏膜组织的阳性表达率分别为 88%、22. 5% ( P <0. 001),与胃癌浸润深度、淋巴结转移、临床分期相关(P<0. 001). FOXD3蛋白在胃癌组织和癌旁正常胃黏膜组织的阳性表达率分别为22%、90% ( P<0. 001 ) ,与胃癌浸润深度、淋巴结转移、临床分期相关( P <0. 001 ). CD44v6 与FOXD3表达呈显著负相关性( rs = - 0. 276, P < 0. 01 ).CD44v6和FOXD3在110例胃癌组织芯片中的表达结果与以上传统切片中的表达情况相似. 结论 FOXD3作为干细胞因子,其与胃癌生物学行为的关系以及与CD44v6的相关性提示FOXD3可能作为胃癌干细胞标记物.%Objective To research the expressions of CD44 v6 and FOXD3 protein in human gastric cancer tissue and the relation with the biologic behavior of gastric carcinoma. Methods The expressions of CD44v6 and FOXD3 in human gastric cancer and gastric normal tissue (100 cases of human gastric cancer,40 cases of gastric normal tis-sues and 110 cases of gastric cancer microarray) were detectd by immunohistochemistry Envision two step method. Results The positive expression rate of CD44 v6 in gastric cancer and nomal gastric tissue were respectively 88%and 22. 5% (P<0. 001) in traditional tissue species,which were related with the depth of infiltration, the transfer of lymph gland and clinical stages ( P<0. 001 ) . In the gastric cancer tissue and normal tissue adjacent to carcino-ma, the expression rates of FOXD3 were respectively 22% and 90% (P<0. 001),which were also related with the depth of infiltration, the transfer of lymph gland and clinical stages (P<0. 001). The relation between the expres-sions of CD44v6 and FOXD3 was significantly nagtively correlated (rs= -0. 276,P<0. 01). The expression results of CD44 v6 and FOXD3 in gastric cancer tissue microarray were similar with that in the traditional pathologic sec-tion. Conclusion As a stem cell,it is possible that FOXD3 serves as a marker of gastric stem cells concerning its relation with the biologic behavior of gastric cancer and its correlation with FOXD3 .