人胃癌SGC-7901/TCF7 L2细胞株的建立及其生物学特性观察

摘要

Objective To establish human gastric cancer SGC-7901/TCF7 L2 cell line and observe its biological characteristics. Methods Stable plasmid transfection technique was used to obtain high TCF7L2 protein expression of gastric cancer cell line SGC-7901/TCF7L2 and idling control SGC-7901 cell lines (SGC-7901/CON). RT-PCR and Western blot were used to detect SGC-7901/TCF7 L2 and SGC-7901/CON two cell lines of TCF7 L2 gene mR-NA and protein expression levels; under the optical microscope,to observe and compare the morphological of two cell lines. Wound healing experiments were used to compare the ability of migration between the two cell lines. Cell counts were detected both cisplatin and carboplatin drug resistance. Tumor-burdened experiments in nude mice were used to compare the ability of tumorigenicity between two cell lines. Results Stably transfected technology successfully established stable SGC-7901/TCF7L2 cell line, which compared with SGC-7901/CON cell line,mRNA and protein expression of TCF7L2 amount were significantly increased; under the optical microscope, SGC-7901/TCF7L2 cell line had morphological diversity,increased pseudopodia and volume, more particulate matter in the cy-toplasm of particulate matter compared with SGC-7901/CON cell line. Migration and tolerance of platinum com-pared with control cell line was significantly enhanced. The tumorigenicity of SGC-7901/TCF7L2 cell line in mice also significantly enhanced. Conclusion TCF7L2 gene can enhance the biological characteristics of malignant gas-tric cancer cell line SGC-7901 . The establishment of SGC-7901/TCF7 L2 cell line provides the basis for the re-search on development and mechanism of gastric cancer,and it is also helpful for the development of targeted drugs.%目的：建立人胃癌 SGC-7901/TCF7L2细胞株,观察其生物学特性。方法使用质粒稳定转染技术,获得高表达TCF7L2蛋白的胃癌细胞株 SGC-7901/TCF7L2及其空转对照组SGC-7901细胞株( SGC-7901/CON)。采用qRT-PCR和Western blot 法分别检测 SGC-7901/TCF7L2和 SGC-7901/CON两细胞株中的TCF7L2 mRNA和蛋白表达水平；光学显微镜下观察并比较两种细胞株的细胞形态；划痕实验比较两者的迁移能力；用细胞计数法分别检测两者对顺铂和卡铂的耐药情况；裸鼠体内荷瘤实验比较两者在体内成瘤的能力。结果经稳定转染技术成功建立稳定的SGC-7901/TCF7L2细胞株,其TCF7L2的mRNA和蛋白表达量均较SGC-7901/CON细胞株明显升高；光镜下SGC-7901/TCF7L2细胞形态较对照细胞形态多样,伪足增多,体积增大,胞质内颗粒物质增多；迁移能力和对铂类的耐受性较对照细胞明显增强；SGC-7901/TCF7L2细胞在小鼠体内的成瘤能力也明显增强。结论 TCF7L2可以增强人胃癌细胞株SGC-7901的恶性生物学特性,SGC-7901/TCF7L2细胞株的建立为胃癌发生发展机制研究及其靶向药物开发提供基础。