外周血间充质干细胞复合富血小板血浆对腱骨界面愈合的影响

摘要

To investigate the effect of peripheral blood mesenchymal stem cells(PB-MSCs) combined with platelet rich plasma(PRP) on tendon-to-bone healing.48 New Zealand rabbits were divided into four groups, operation was performed to reconstruct anterior cruciate ligament, the gel, PRP gel, PB-MSCs gel and PRP+PB-MSCs gel were injected into femoral tunnel of each group.At 4th, 8th and 12th week after ACL reconstruction, 4 rabbits were randomly executed in each group, HE staining was used to detect histological characteristics.Compared with control group, PRP group,PB-MSCs group and PRP+PB-MSCs group had higher histomorphometric scores of three time points, among which the PRP+PB-MSCs group had the highest score(P<0.01).The resluts demonstrated that PB-MSCs and PRP could promote the healing of tendon-to-bone,and PB-MSCs had a synergistic effect with PRP.%探讨兔外周血间充质干细胞(PB-MSCs)复合富血小板血浆(PRP)对腱骨愈合的作用效果.48只新西兰兔分成4组,行前交叉韧带重建术,分别向各组股骨道内注入凝胶、PRP凝胶、PB-MSCs凝胶及PRP+PB-MSCs凝胶.术后4、8、12周各组随机处死4只实验兔,对标本行HE染色,观察腱骨界面组织形态学特点.与对照组相比,PRP组、PB-MSCs组及PRP+PB-MSCs组3个时间点组织形态学评分更高,其中PRP+PB-MSCs组评分最高,差异有统计学意义(P<0.01). 说明PRP、PB-MSCs均能促进腱骨愈合,并且PB-MSCs与PRP具有协同作用.