白细胞介素-1β在类青光眼引流阀植入术后瘢痕化中的作用

摘要

Objective To observe interleukin-1β ( IL-1β) expression on scarring after implantation of glaucoma drainage valve-alike, and to explore its effect on scarring. Methods Thirty New Zealand white rabbits were ran-domly assigned tosilicone group and new material group with 15 rabbits in each group, which implanted silicone and PMMA (Polymethyl methacrylate) coated Parylene C respectively under the conjunctiva. In the two groups, the whole tissues from conjunctiva to sclera were collected at 1 week, 4 week and 8 week respectively after surgery. The expression of inflammatory cell infiltration and fibroblast proliferation in each group were observed by HE stai-ning, and fibroblasts were counted by advanced microscope. Western blot and immunohistochemistry were used to detect IL-1β expression and location in tissues. The correlation coefficient was statistical analyzed between IL-1β protein and fibroblasts. Results According to HE straining result, silicone group showed significantly higher ex-pression of inflammatory cell infiltration and fibroblast proliferation compared with the new material group. Accord-ing to Western blot and immunohistochemistry results, the concentrations of IL-1β were higher in silicone group than that in new material group at each time point ( P ＜0.05 ) . According to immunohistochemistry result, in-creased IL-1β was located around the proliferated fibroblasts on surface of the sclera. Correlation coefficient showed that the expression of IL-1β was positively correlated with the fibroblasts in 4 week and 8 week tissues ( P＜0.05) . Conclusion IL-1β may promote the formation of scars after implantation of glaucoma drainage valve.%目的 检测白细胞介素-1β( IL-1β)在类青光眼引流阀植入术后瘢痕组织中的表达水平,明确其在瘢痕形成中的作用.方法 将30只新西兰大白兔随机分为硅胶组和新材料组,分别于球结膜下植入硅胶和聚一氯对二苯( Parylene C)涂层包裹的聚甲基丙烯酸甲酯(PMMA);于术后1、4、8周取球结膜至巩膜全层组织,采用苏木精-伊红( HE)染色检测组织中炎细胞浸润和成纤维细胞增生情况,于高倍镜下计数成纤维细胞;免疫组化检测IL-1β蛋白的表达及组织中的定位;Western blot法检测IL-1β蛋白表达水平;并统计分析IL-1β蛋白与成纤维细胞的相关系数.结果 HE 染色显示:硅胶组炎细胞浸润、成纤维细胞增生数量明显高于新材料组;Western blot及免疫组化检测术后1、4及8周硅胶组的IL-1β蛋白的表达明显高于新材料组(P＜0.05);免疫组化显示:IL-1β主要位于近巩膜面增殖的成纤维细胞周围.相关性分析结果表明:4、8周组织中IL-1β的表达水平与成纤维细胞呈正相关性 (P＜0.05).结论 IL-1β可能在青光眼引流阀植入术后的瘢痕化形成中起促进作用.