以香水百合为试验材料,采用四因素四水平L16(44)正交设计方法,研究了6-苄基氨基嘌呤(6 -BA)、赤霉素(GA3)、吲哚丁酸(IBA)3种植物生长调节剂的不同浓度及不同浸球时间对香水百合光合色素及相关酶活性的影响.结果表明,不同时期,各处理叶绿素a、叶绿素b、叶绿素a+b的含量均高于对照,且与对照差异显著,呈先上升后下降的趋势,在现蕾期或初花期达到最大值,GA3 150 mg/L、IBA 40 mg/L、6 -BA 60 mg/L、浸球40 min时叶绿素含量最高.超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性增强,GA3 150 mg/L、IBA 40 mg/L、6- BA 60mg/L、浸球40 min时二者均维持在较高水平.丙二醛(MDA)的含量低于对照,且上升趋势减缓,GA3 100～150 mg/L、IBA 40～60 mg/L、6- BA 60 mg/L、浸球10～40 min时MDA含量最低.综合分析表明,在所有处理中,以150 mg/L GA3+40 mg/L IBA+60 mg/L 6- BA组合和40 min浸球处理的合理搭配效应为最佳.%Effects of plant regulators on photosynthetic pigment content and enzyme activity in leaves of Lilian casa blanca (Lily) were studied using an orthogonal design [L16(44)] of 6-BA, GA3 , IBA, and bulb soaking time. Experimental data was analyzed by ANOVA and correlation analysis. In different periods,the contents of chlorophyll a, chlorophyll b, and chlorophyll a+b in leaves were significantly higher than those in the CK. During the whole growth period, these photosynthetic pigment contents were highest when GA3 was 150 mg/L, IBA was 40 mg/L, 6-BA was 60 mg/L and soaking time was 40 min. They increased to maximum at the squaring stage or initial bloom stage, and then decreased slightly or strongly. The activity of superoxide dismutase (SOD) , and of catalase (CAT) was enhanced. Both SOD and CAT stayed at a high level when GA3 was 150 mg/L, IBA wais 40 mg/L, 6-BA was 60 mg/L and soaking time was 40 min. However, the content of maloaldehyde (MDA) in leaves was lower than that of the control, and the upward trend of MDA in leaves became less. The MDA content was lowest when the experimental condition was GA3 100-150 mg/L, IBA 40-60 mg/L, 6-BA 60 mg/L and soaking time was 40 min. Analyses showed that the best combination of plant regulators was GA3 150 mg/L, IBA 40 mg/L, and 6-BA 60 mg/L with a soaking time of 40 min.
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