微透析取样和微柱液相色谱电化学检测法测定大鼠脑纹状体中的5-羟色胺

摘要

AIM: To develop a sensitive method for determination of serotonin in biological samples.METHODS: A combination of microdialysis sampling and microbore liquid chromatography with electrochemical detection (LCEC)was established. RESULTS: Changes of serotonin in fg or pg in microdialysates from brain striatum of the free moving rat were easily determined. CONCLUSION: This developed method was useful for living animal research. Serotonin level in corpus striatumhealthy rats was quite stable.%目的:建立一个测定生物样本中5-羟色胺的灵敏方法.方法:将微透析取样和微柱液相色谱电化学检测(LCEC)有机结合,建立灵敏的分析方法.结果:很容易地测定了自由活动大鼠脑纹状体透析液中fg-pg的5-羟色胺变化.结论:本法对生物活体研究很有用.健康大鼠脑纹状体内5-羟色胺水平是相当稳定的.