花粉提取物EA-10,P5对慢性前列腺炎及其合并不育症的作用

摘要

目的:探讨EA-10,P5治疗慢性前列腺炎及其合并不育症的药物机理.方法:采用生物化学法测定MDA、SOD及N0,原子吸收分光光度法测定Zn2+浓度.结果:慢性前列腺炎组治疗前较对照组MDA、NO明显升高,SOD、Zn2+浓度显著降低;治疗后MDA、N0明显降低,Zn2+浓度显著升高,但SOD无显著性变化(P＞0.05).而慢性前列腺炎合并不育症组治疗前较对照组MDA、NO明显升高,SOD、Zn外浓度显著降低;治疗后MDA、NO明显降低,SOD、Zn2+浓度显著升高,同时精子的活动率、死亡率较治疗前明显好转.结论:氧自由基与慢性前列腺炎的发生发展及好转有着密切关系;EA-10,P5用于治疗慢性前列腺炎及其合并不育症的药物机理与氧自由基的改变有关.%AIM: To determine the drug action mechanismof pollen extract EA-10, P5 on the treatment of chronic prostatitis(CP) or infertility with CP. METHODS: Malondiadehyde (MDA), superoxide dismutase (SOD), and nitrogenmonoxide (NO) were measured by biochemical assay, and zinc content was assayed by atomical spectrophotographyin the pre-treatment and post-treatment of CP or infertility with CP. RESULTS: Compared with control group,leukocytes in expressed protatic secretion (LEPS), MDA, and NO were increased, and zinc content and SOD weredecreased significantly in the pre-treatment of CP. After the treatment, LEPS was improved, and MDA and NOwere reduced, while zinc content were increased apparently and the alteration of SOD was not evident (P＞0.05).In the pre-treatment of infertility with CP, LEPS, MDA, NO, sperm viability, and seminal leukocytes were obvi-ously higher and seminal plasma SOD, zinc content, and sperm motility were obviously lower than those in controlgroup. After the treatment, LEPS, sperm motility, and sperm viability were improved, MDA, NO, and seminalleukocytes were decreased, SOD and zinc content were increased markedly. CONCLUSION: There was inter-correlation between oxygen free radicals (OFR) and occurrence, development, and recovery of CP; Change ofOFR may be involved in the drug action mechanism of EA-10, P5 in the treatment of CP or infertility with CP.