粒细胞-巨噬细胞集落刺激因子和干扰素-γ诱导脐血嗜碱粒细胞表达人类白细胞抗原Ⅱ类分子

摘要

目的:探讨嗜碱粒细胞是否可表达人类白细胞抗原(HLA)Ⅱ类分子.方法:采用密度梯度离心和免疫磁珠方法分离和纯化脐血嗜碱粒细胞,通过体外培养及粒细胞-巨噬细胞集落刺激因子(GM-CSF)和干扰素(IFN)-γ刺激20-60小时后,用流式细胞仪分析HLAⅡ类分子表达的情况.结果:嗜碱粒细胞的纯度≥83.5%,在GM-CSF 10μg/L或IFN-γ 100kU/L刺激后,其膜表面可表达HLAⅡ类分子,表达百分率在刺激后20小时较高,分别为10.2%±2.1%和11.3%±1.0%.结论:嗜碱粒细胞具有表达HLAⅡ类分子的潜在能力.%AIM: To determine whether basophils expressed human leucocyte antigen (HLA) class Ⅱ molecules. METHODS: Basophils in umbilical cord blood were separated and purified with methods of density gradient centrifugation and immunomagnetic microbeads. The isolated basophils were cultured in RPMI-1640 plus 10 % fetal caff serum at 37 ℃ in a humidified atmosphere with 5 % CO2 and stimulated with granulocyte-macrophage colonystimulating factor (GM-CSF) or interferon ( IFN)-γ for 20-60 h. The expression of HLA class Ⅱ molecules on basophils was measured with fluorescence activated cell sorter (FACS). RESULTS: The purity of isolated basophils was ≥83.5 %. After treated with GM-CSF 10 μg/L or IFN-γ 100 kU/L, the expression of HLA class Ⅱ molecules became detectable on membranous surface and at a higher level at 20 h, the percentage of expression was 10.2 % ± 2.1% and 11.3 % ± 1.0 %,respectively. CONCLUSION: Basophils possessed the potency of HLA class H molecular expression.