含有柴胡的植物复方药KY88 LIVER LIV0刺激人类淋巴细胞产生细胞因子

摘要

AIM: Compounds containing Bupleurum possess immunomodulating effects. KY88 LIVER LIVO (KY88) is ablend of such compound. The aim of this study is to investigate the effects of KY88 on the production of cytokinesby lymphocytes in vitro. METHODS: Seventy Sprague Dawley rats were used of which 40 were orally fed with 4mg purified KY88 for 35 d. Normal human lymphocytes were isolated and cultured in standard conditions. Theculture medium was collected at zero and 72 h after the KY88 treatment. The cytokines, including interleukin-1 β(IL-1β), IL-2, IL-4, IL-6, tumor necrosis factor-α (TNF-α), and interferon-γ, were measured by ELISA kits.RESULTS: TNF-α levels in the supernatant of cultured human lymphocytes significantly increased after the treat-ment of PHA and KY88. The mean levels were (855±251), (399± 145), and (176±49) ng/L after the treatment withKY88 at the concentrations of 10, 1 and 0.1 g/mL respectively. However, the level in the control group withoutspecific treatment was only (68±4) ng/L. The difference between KY88 10 g/mL and control groups was signifi-cant (P＜0.05). All other cytokines did not show significant variations between KY88 and the control groups. KY88may regulate the immune function through the induction of TNF-o expression.