野生鲤鱼耳石中蛋白质的提取与表征

摘要

通过对北京密云水库的野生鲤鱼的微耳石和星耳石中的蛋白质进行提取,分别得到了3种基质蛋白,根据其在提取过程中溶解性的不同,分为水可溶蛋白(WSP)、酸可溶蛋白(ASP)和酸不可溶蛋白(AIP).通过高效液相色谱、X射线衍射、红外光谱分析和氨基酸成分分析等方法分别对3种基质蛋白进行了表征,发现微耳石和星耳石中3种基质蛋白特征存在差异,并初步推断蛋白质特征的差别与碳酸钙晶体在微耳石和星耳石中的不同晶型有关.%The proteins in lapillu and asteriscu of wild carp from the Miyun Reservoir in Beijing were extracted. According to the difference in solubility, the proteins can be classified into three groups, namely, water soluble protein( WSP), acid soluble protein( ASP) and acid insoluble protein(AIP). Using high performance liquid cliromatography(HPLC), X-ray diffraction(XRD) and Fourier transformation infrared spectra (FTIR), the authors characterized the sorted proteins from different otoliths. It is found that the three types of proteins in lapillus and asteriscus have different characteristics, and such differences may be responsible for different kinds of crystalline state in calcium carbonate.