本研究利用线粒体细胞色素氧化酶亚单位I (COI基因) 片段对伊蚊族117个蚊种进行了系统发育关系的研究.分别使用简约法、似然法以及贝叶斯法重建了系统发育树, 并用Shimodaira-Hasegawa检验法从3个系统发育树中选择出最佳基因树.与近年来根据形态特征对伊蚊族重新分类的研究相比, 本研究中的结果显示, 支持伊蚊族新分类研究结果中的阿蚊属阿蚊亚属、 Bothaella属、 Collessius属 Alloeomyia亚属、领蚊属Jarnellius属,骚扰蚊属Culicelsa和Woodius亚属、'Ochlerotatus'、覆蚊属Heteraspidion亚属的单系群地位.然而, 并不支持阿蚊属、 Downsiomyia属、 Hulecoeteomyia属、骚扰蚊属Rusticoidus亚属、 Phagomyia属、Psorophora属和覆蚊属的单系群地位.另外, 分子数据显示骚扰蚊属中的部分成员较为原始, 并且Ochlerotatus theobaldi显示出与其他所有蚊种的姐妹群关系; 骚扰蚊属和覆蚊属为并系群.%The phylogenetic relationships among 117 species in the tribe Aedini were analyzed and discussed based on the sequence variation of a 407 base pair (bp) fragment of the cytochrome oxidase I gene (COI gene). Phylogenetic trees were reconstructed on the basis of parsimony, maximum likelihood and Bayesian methods, and Shimodaira-Hasegawa tests were used to determine the best tree using RELL approximations. Despite differences in the taxa examined, the results were generally consistent with the recent morphological taxonomic revision on this group. The monophyly of the following genera and subgenera was corroborated: Armigeres ( subgenus Armigeres ), Bothaella, Collessius ( subgenus Alloeomyia ), Heizmannia, Jarnellius, Ochlerotatus ( subgenera Culicelsa and Woodius ), ‘Ochlerotatus’, Stegomyia ( subgenus Heteraspidion). However, the monophyly of the following genera and subgenera was not supported: Armigeres, Downsiomyia, Hulecoeteomyia, Ochlerotatus (subgenus Rusticoidus), Phagomyia, Psorophora and Stegomyia. In addition, the molecular data indicate that the genus Ochlerotatus (in part) appears to be the most primitive group and that Ochlerotatus theobaldi be a sister-species to all other taxa. Two genera, Ochlerotatus and Stegomyia, were found to be polyphyletic.
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