海洋酸化对海水青鳉胚胎骨骼发育的影响

摘要

In this study,the impact of ocean acidification on the skeletal development in embryonic marine medaka was investigated.The seawater carbonate system in the water was maintained stable by aerating with ambient air (450×10-6 CO2 )and CO2-enriched air (1 160×10-6 or 1 783×10-6 CO2 ).Newly fertilized medaka eggs were exposed to three levels of pCO2/pH (8.14,7.85 and 7.67)until to the main hatch occurring.Skeletons of 30 new-hatched larvae from each CO2 treatment were cleared,stained and photographed.Lengths of well stained 28 skeletal elements for ecah fish was measured using digital photograph and analyzed by image analysis software.Results showed that,the effects of exposure to elevated CO2 concentrations on the length of representative skeletal elements were not significant.It suggested that the skeletal development of marine medaka would not be seriously affected by the changes in CO2 concentrations that are predicted to occur over the next 100 to 200 years.%本文在实验室模拟近期海洋酸化水平，对海洋酸化对海水青鳉鱼（Oryzia melastigma ）胚胎骨骼发育的影响进行了初步研究。实验中，通过往实验水体中充入一定浓度 CO2气体酸化海水。对照组 CO2分压为450×10－6，两个处理组 CO2浓度分别为1160×10－6和1783×10－6，对应的水体 pH 值分别为8．14，7．85和7．67。将海水青鳉鱼受精卵放入实验水体中至仔鱼孵化出膜，对初孵仔鱼经骨骼染色、显微拍照，挑取了仔鱼头部、躯干及尾部骨骼染色清晰的28个骨骼参数的长度进行了显微软件测量及数据统计分析。结果发现，酸化处理对实验鱼所测量的骨骼长度影响均不显著。因此推测，未来100～200年间海洋酸化对海水青鳉鱼的胚胎及初孵仔鱼的骨骼发育没有显著影响。