链脲佐菌素诱导大鼠糖尿病合并脑缺血损伤模型的建立

摘要

目的：建立稳定的大鼠糖尿病合并局灶性脑缺血损伤模型，并对有关建模成功的标准进行探讨与分析。方法：40只健康雄性SD大鼠随机分为正常饲养假手术组、正常饲养脑缺血再灌注损伤模型组、糖尿病脑缺血再灌注损伤模型组，糖尿病模型组一次性腹腔注射（ip）链脲佐菌素（streptozotocin，STZ）60 mg·kg-1建立1型糖尿病大鼠模型，分别在造模1，4周后检测各组大鼠的体质量和空腹血糖。在ip STZ 4周后采用线栓法制备大鼠局灶性脑缺血再灌注模型，缺血30 min，复灌注24 h，进行神经功能评分，测定脑梗死体积并计算梗死体积百分比。结果：腹腔注射STZ 4周后，糖尿病模型组与正常组大鼠相比，体质量显著下降（P<0.001），血糖值显著升高（P<0.001）。缺血复灌注损伤手术后，糖尿病模型组与正常组大鼠相比，再灌注损伤加重，脑梗死体积百分比显著增加（P<0.001）。模型成功率为73%。结论：本实验方法制备的大鼠糖尿病合并局灶性脑缺血损伤模型成功率较高，具有较好的重复性和稳定性。%Objective:To establish a reliable model of focal cerebral ischemia injury in diabetic rats,and to explore the criteria for successful modeling. Methods: Forty healthy male SD rats were randomly divided into three groups: a normal sham-operation group,a normal cerebral ischemia-reperfusion injury group and a group of diabetes combined with cerebral ischemia-reperfusion injury. Type I diabetes was induced by an intraperitoneal injection of streptozotocin(STZ,60 mg·kg-1). The body mass and the level of fasting blood glucose in each group were measured on Weeks 1 and 4 after STZ injection. A focal cerebral ischemia-reperfusion model was prepared by a thread occlusion method four weeks after STZ treatment. The scores of neurological function were assessed and the percentage of infarct volume were calculated after 30 min ischemia followed by 24 h of reperfusion. Results: The body mass (P<0.001)was reduced and the fasting blood glucose(P<0.001)was increased significantly in diabetic rats than those in normal rats four weeks after STZ injection. The percentage of infarct volume(P<0.001)was increased more significantly and the brain injury appeared more severe in diabetic rats than in normal rats after reperfusion. The success rate of this model was 73%. Conclusion: In this study,we developed a model of focal cerebral ischemia injury in diabetic rats that demonstrates a high success rate and good reliability and stability.