七氟烷增强单个培养鼠背根神经节细胞γ-氨基丁酸调控的氯电流

摘要

The whole-cell patch clamp technique and "Y-tube" method were applied to evaluate the effects of sevoflurane (from 0. 3×10-3to 3×10-3 mol/L) on chloride current induced by bath utilization of 3×10-6 mol/L γ-aminobutyric acid (GABA) in the single-cultured rat dorsal root ganglia neurons. Experimental data demonstrated that when peak amplitude of chloride current induced by 3 × 10-6 mol/L GABA was considered as 100 % in the presence of 0. 38×10-3, 0. 76×10-3, 2. 28×10-3, 3. 04×10 -3 mol/L sevoflurane peak amplitude of chloride current was rose to (149±25) %, (203±-27) %, (327±79) %, (331±109) %, (243±71) % correspondingly. This finding suggests that sevoflurane, at concentrations relevant clinical anesthesia, can enhance GABA-mediated chloride current in sensory neurons.%采用膜片钳全细胞记录和"Y型管"技术，观察七氟烷(0．38×10-3～3×10-3mo1／L)对单个培养SD鼠背根神经节细胞3×10-6mol/Lr-氪基丁酸(GABA)调控的氯电流影响，实验结果发现，0．38×10-3，0．76×l0-3，1．52×10-3，2．28×10-3，3．04×10-3mol/L七氟烷分别增强氯电流峰值高度至对照值的(149±25)％，(203±27)％，(327±79)％，(331±109)％，(243±71)％。实验结果提示，临床麻醉浓度的七氟烷能增强培养鼠背根神经节细胞3×10-6mol/LGABA调控的氯电流。