从妊娠人胎盘绒毛组织克隆HLA-G1 cDNA

摘要

为系统研究HLA-G1分子的生物学功能，采用RT-PCR技术，从人流的胎盘绒毛组织中提取总RNA，经过逆转录合成HLA-G1的全长cDNA，插入到真核表达载体pcDNA3中，构建重组表达质粒pcDNA3-HLA-G1；转化大肠杆菌DH5α，筛选其阳性克隆株，经酶切、PCR及测序鉴定，证实HLA-G1分子的重组真核表达载体已构建成功。%In order to systematically study the function of HLA-G1 molecule，total RNA was extracted from the first trimester trophoblast and the full length HLA G1 cDNA was prepared by using the technique of RT-PCR. Recombinant of HLA-G1 cDNA and plasmid pcDNA3 was constructed，and then transformed into E. coli DH5α. The recombinant was confirmed by restriction enzyme analysis and PCR analysis. The result of DNA sequencing showed that the pcDNA3-HLA-G1 recombinants had successfully been constructed.