贞清方通过抑制活性氧产生改善2型糖尿病大鼠勃起功能

摘要

目的 探索贞清方能否通过减少活性氧(ROS)的产生来改善糖尿病引发的勃起功能障碍(ED).方法 30只8周龄雄性SD大鼠,随机分为对照组(Con组),糖尿病ED组(DM组),糖尿病ED+贞清方组(ZQR组),其中DM 组和ZQR组均采用高糖高脂喂养4周后行腹腔单次小剂量(30 mg/kg)注射链脲佐菌素(STZ)诱导2型糖尿病模型,并于10周后使用阿扑吗啡(APO)筛选2型糖尿病ED大鼠.ZQR组使用贞清方4 g/(kg·d)灌胃,DM组和Con组给予等量的生理盐水灌胃.4周后测各组大鼠血糖、三酰甘油(TG)和总胆固醇(TC)、平均动脉压(MAP)、阴茎海绵体内压(ICP)、丙二醇(MDA),通过Western blot检测内皮型一氧化氮合酶(eNOS)和神经型一氧化氮合酶(nNOS)的蛋白表达.结果Con组和ZQR组大鼠血糖、TC、TG、和MDA水平均明显低于DM组(均 P<0.05);Con组和ZQR组的最大阴茎海绵体内压/平均动脉压(maxICP/MAP)明显高于DM 组(P<0.05);Western blot 结果显示eNOS和 nNOS在Con组和ZQR组的表达明显高于DM组.结论 贞清方可以通过抑制活性氧的产生来改善2型糖尿病大鼠的勃起功能障碍.%Objective To investigate whether Zhenqing Recipe(ZQR)could ameliorate diabetes-related erectile dysfunction (ED)by reducing the reactive oxygen species(ROS)production.Methods Thirty 8-week-old healthy male SD rats were random-ly divided into three groups:control group(group Con),diabetic erectile dysfunction group(group DM),and diabetic erectile dysfunction treated with Zhenqing Recipe group(group ZQR).Type 2 diabetic rat model was induced by intraperitoneal injection of small-dose streptozotocin after feeding with high glucose and high fat diets for four weeks.We screened out diabetic erectile dysfunction rats by apomorphine 10 weeks later.The course of treatment continued four weeks.And then blood glucose,triglyc-eride(TG),cholesterol total(TC),mean arterial pressure(MAP),intracavernouspressure(ICP),expression levels of nNOS and eNOS protein were measured by Western blotting.Results Compared with the control group,the levels of blood glucose and MDA were remarkably increased in diabetic rats.ZQR remarkably reduced levels of blood glucose and MDA of diabetic rats.Compared with the control group,the ratio of maximum ICP-to-MAP(maxICP/MAP)was significantly decreased in diabet-ic ED rats.ZQR improved erectile function of the diabetic ED rats.Expression levels of nNOS and eNOS protein were reduced as compared to those of control rats.ZQR significantly enhanced their expression levels in diabetic rats.Conclusion ZQR can a-meliorate type 2 diabetic ED by reducing the ROS production.