低频电刺激对中国树鼩死亡后脏器中激素水平影响

摘要

目的：探索低频电刺激对中国树鼩死亡后脏器中激素水平的影响。方法给予中国树鼩低频电刺激，分别于死亡后0、3、6、12、18、24、36 h和72 h取甲状腺、肝、脾，用放射免疫法测定内皮素（ endothelin， ET）、心钠素（ atrial natriuretic factor， ANF）、血栓素（ thromboxane， TX）水平；剥取死后0 h中国树鼩中脑腹侧背盖区（ ventral tegmental area，VTA）检测c－fos表达。结果电刺激后，中国树鼩尸体脏器中内皮素、心钠素、血栓素水平比对照组显著升高，其水平随死亡后时间的延长而降低；VTA c－fos表达明显增强。结论低频电刺激能引起中国树鼩脏器中激素的合成、释放和脑组织c－fos表达。%Objective To study the effect of low frequency electrical stimulation on hormone levels in organs of Chinese tree shrews after death.Methods Giving Chinese tree shrews low frequency electrical stimulation.At 0 h, 3 h, 6 h, 12 h, 18 h, 24 h, 36 h and 72 h after death, the thyroid, liver, spleen were taken,and the levels of endothelin (ET), atrial natriuretic factor( ANF) , thromboxane ( TX) were determined by RIA method.At 0 h after death, midbrain ventral tegmental area ( VTA) of Chinese tree shrews was taken to detect the c-fos expression.Results After electrical stimula-tion, ET, ANF, TX levels in the cadaver organs and VTA c-fos expression of Chinese tree shrews were significantly in-creased than in the control group.The contents were decreasing with the time after death.Conclusions Low frequency e-lectrical stimulation can induce the synthesis and release of hormones in organs and c-fos expression in brain tissue of Chi-nese tree shrews.