PM2.5对雌鼠生殖内分泌水平和妊娠结局的影响

摘要

目的 研究PM2.5对雌性大鼠生殖内分泌激素水平和妊娠结局的影响.方法 30只雌鼠随机分为对照组(生理盐水)、低剂量PM2.5组(1.5 mg/kg)和高剂量PM2.5组(37.5 mg/kg).PM2.5暴露10 d,采集孕前血液后合笼,妊娠第19天时,处死大鼠,采集并分离血清,用ELISA试剂盒测定妊娠前后血清中雌二醇(estradiol,E2)、孕酮(progesterone, PROG)、绒毛膜促性腺激素(chorionic gonadotropin, CG)、黄体生成素(luteinizing hormone, LH)和促卵泡生成素(follicle-stimulating hormone, FSH)激素水平;剖宫观察胎鼠情况.结果 对照组、低剂量组和高剂量组的活胎率分别为90.77%、59.49%和60.27%.与对照组相比,低剂量组和高剂量组胎鼠的活胎率均明显降低(P<0.05).与对照组相比,PM2.5能够明显降低各暴露组雌鼠妊娠前后血中E2、PROG、CG和LH水平(P<0.05).与对照组相比,低剂量PM2.5暴露组雌鼠妊娠前血中FSH变化不明显(P>0.05),但高剂量组血中FSH明显降低(P<0.05).结论 PM2.5可能通过影响内分泌激素分泌水平而影响妊娠结局.%Objective To study the effects of PM2.5 on reproductive hormone levels and pregnancy outcome in female rats.Methods Thirty healthy 4-week old female Sprague-Dawley rats were randomly divided into the control group (normal saline), low dose of PM2.5(1.5 mg/kg) group and high dose of PM2.5 (37.5 mg/kg) group.After the blood samples were collected, the animals were exposed to PM2.5for 10 days, and then the rats were mated.On the 19th day of pregnancy, the rats were sacrificed for pregnancy outcome observation and blood samples were collected for hormone test.The blood hormone levels were detected using an ELISA kit.Results The live fetus rates in the control, low dose PM2.5 and high dose PM2.5 groups were 90.77%, 59.49% and 60.27%, respectively (P<0.05).The live fetus rates in the low dose PM2.5 and high dose PM2.5 groups were significantly lower than that in the control group (P<0.05).PM2.5decreased the levels of E2, PROG, CG and LH (P<0.05), compared with that in the control group.Although the blood levels of FSH were not significantly different between the low dose and control groups (P>0.05), the level of FSH in the high dose group was significantly decreased (P<0.05).Conclusions PM2.5may affect pregnancy outcome through influencing the hormone levels.