The pathogen Hyriopsis cumingii Plague Virus isolated from the diseased H. cumingii Lea has been artificially transferred to the healthy H. cumingii Lea. The infected H. cumingii Lea has displayed some typical pathological changes. Observations by histopathology have showed that most of organs including the digestive gland, stomach and intestine are the primary targets of the plague virus. Lesions have been observed, accompanied with a number of empty areas, cell vacuolization and some cells falling-off. Arenavirus-like particles have been detected by using transmission electron microscopic (TEM) examination on investigated organs of the artificially infected bivalve mussel Hyriopsis cumingii. Virions are spherical with a size of 120 nm in diameter, and the outer layer of the intact arenavirus-like particles have small protuberance and moderate electron-dense nucleicapsid. Under electronic microscope, the most obvious cellular pathologic feature is the large vacuole areas of cytoplasm, and there is the reduction or devoid of organelles in rnthe cytoplasm. Varying degrees of denaturalization and necrosis have been found in the intestinal mucosa epithelial cells.We have also observed that in cytopathic cells of digestive gland, the nuclei has been discovered to be amorphous and rnhypertrophied. There are denaturalization and necrosis in the epithelial cell of gill and the nucleus membrane of granulocytes have been dissolved.%从自然发病的病蚌中提取出病毒,经人工感染健康三角帆蚌后呈现出典型的病理变化.组织学观察显示消化腺、胃、肠、外套膜、斧足和鳃等均出现大量细胞空泡化、部分细胞肿大、上皮细胞排列不紧密,结缔组织不完整,部分细胞溶解甚至脱落等,表明它们均为病毒损害的主要靶器官.透射电镜观察显示大量砂样病毒存在于患病三角帆蚌不同器官中.病毒粒子为直径约120 nm的圆球形,表面具有明显的纤突,无囊膜结构.被感染细胞呈现明显的病理变化,包括细胞质大片空泡化,细胞器相对减少或缺失,细胞核电子密度加深,肠黏膜上皮细胞不同程度的变性和坏死,消化腺细胞核变形肿大,鳃小片上皮细胞坏死脱落,颗粒细胞的核膜溶解等.
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