To investigate effects of re-feeding following fasting on plasma glucose, total protein, triacylglycerol, total cholesterol, low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) contents in cobia fasted for 24h, six diets were formulated to contain 0% (control), 6%, 12%, 18%, 24% and 30% starch, respec-tively. The results showed that the serum glucose levels of cobia after re-feeding with each diet elevated from 0h to 5h and then started to decline till 7h that the glucose levels were similar to those at 0h. Different dietary starch and post-prandial time did not significantly influence the total protein levels in serum. The triacylglycerol levels in serum ele-vated from 0h to 5h and then began to decline. At 7h, the levels were similar to those at 0h. In the control group, the concentration of cholesterol in serum increased from 0h to 2h and then decreased from 2h to 7h that reached to fasting level. In other groups, the serum cholesterol levels increased from 0h to 5h and then began to decline; then, it also reached to fasting level at 7h. The levels of LDL-C increased from 0h to 5h and then began to decline. At 7h, the levels of LDL-C declined to the basal level. However, different dietary starch and postprandial time did not significantly in-fluence the levels of LDL-C in serum. In the group of 24%, the levels of HDL-C at 5h were significantly higher than those at 0 and 24h. No significant differences were found for the levels of HDL-C among other groups. In conclusion, the glucose levels increased and then declined. The peak glucose levels increased with the increase of dietary starch level up to 12% and remained nearly the same thereafter. The levels of triacylglycerol and cholesterol in serum increased after re-feeding, but there were no significant differences in the LDL-C and HDL-C levels in serum after re-feeding.%实验旨在研究经过10周投喂不同淀粉含量的饲料的养殖实验后,禁食1d后再投喂对170 g军曹鱼血清生化指标的影响。饲料以鱼粉为蛋白源,鱼油、豆油、大豆卵磷脂为脂肪源,分别添加0%(对照组)、6%、12%、18%、24%和30%的小麦淀粉(以微晶纤维素调平),养殖实验期间每天饱食投喂2次。结果表明,饥饿再投喂饲料后各处理组军曹鱼血糖含量均在(0—5)h 升高,在5h时达到峰值,并显著高于其他时间组,摄食5h 后开始下降,7h 时基本恢复到投喂前水平；血清总蛋白含量在不同处理组以及同一处理组的不同时间点上均没有显著差异；血清中甘油三酯含量在(0—5)h显著升高,5h后其含量开始下降,7h时基本恢复到投喂前水平。其中0%、6%和12%组,摄食5h后其甘油三酯含量显著高于摄食前与摄食后7h和24h,而在18%、24%和30%组中,其含量无显著差异；0%淀粉组血清中胆固醇含量在(0—2)h 显著升高,2h 时达到峰值,在(2—7)h呈下降趋势,之后趋于平稳。其他处理组都是在(0—5)h升高,5h后开始下降,在7h时基本恢复到投喂前水平。18%—30%组,摄食后5h血清中胆固醇含量显著高于摄食前与摄食后24h。军曹鱼血清中低密度脂蛋白-胆固醇在(0—5)h显著升高,摄食5h后其含量开始下降,7h时基本恢复到投喂前水平,但各组不同时间点间均无显著性差异。而血清中高密度脂蛋白-胆固醇含量除在24%添加组中,摄食后5h显著高于摄食前(0h)及投喂结束后24h处理组,其他处理之间无显著差异。综上所述：170 g军曹鱼禁食再摄入不同含量的小麦淀粉后其血清中血糖含量先升高后降低,随着饲料中糖添加量的增加,其峰值先增加,后趋于平稳。军曹鱼血清甘油三酯和胆固醇的含量会升高,对其血清中低密度脂蛋白-胆固醇及高密度脂蛋白-胆固醇影响不显著。
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