为了解淡水贝类性别调控与分化机制,课题组建立了池蝶蚌(Hyriopsis schlegelii)性腺转录组,在转录组库中,存在β-连环蛋白(β-catenin)基因序列.实验对池蝶蚌β-catenin基因进行验证,采用RACE技术克隆其cDNA全长,命名为Hsβ-catenin.该序列全长4386 bp,5′-非编码区为162 bp,3′-非编码区为1758 bp,开放阅读框为2466 bp,编码821个氨基酸;该蛋白结构域主要由12个ARM重复序列组成;二级结构中,α-螺旋占47.75％,β-折叠占1.22％,随机卷曲占51.04％;三级结构中含大量α-螺旋且为右手超螺旋,构成ARM结构域;系统进化树分析表明,Hsβ-catenin与软体动物聚为一支,然后与昆虫类聚为一支.实时荧光定量PCR(qRT-PCR)检测显示,Hsβ-catenin在肠中表达量最高,其次是斧足和精巢.Hsβ-catenin基因在12月龄和36月龄表达量较高,且在36月龄表达量最高,表明其可能参与池蝶蚌的性别调控与分化作用.%In order to explore the mechanism of sexual development of freshwater shellfish, we established a gonadal transcriptome of Hyriopsis schlegelii. Based on the transcriptome library, this study investigated the β-catenin. The full-length cDNA was cloned by RACE PCR, which named Hsβ-catenin. The full length of Hsβ-catenin cDNA was 4386 bp that contained an open reading frame (ORF) of 2466 bp coding a protein of 821 amino acids. The protein was mainly composed of 12 ARM repeat sequences. In the secondary structure, the α-helix accounted for 47.75％ and the β-folding accounted for 1.22％ were predicted. The tertiary structure had many α-helixs, namely ARM domain. Hsβ-catenin gene was clustered with homologous genes from soft animal in the phylogenetic tree. Hsβ-catenin expressed highly in intes-tine and modestlyin foot and testis. The expression level of Hsβ-catenin was high at the age of 12-month-old (gender differentiation) and 36-month-old (gonad mature), indicated that it might be involved in sexual development.
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