利用RAPD-PCR方法鉴定我国烟粉虱的生物型

摘要

Twenty-three Bemisia tabaci (Gennadius) populations on vegetables, ornamental plants and weeds were collectedfrom 23 different cities of 14 provinces in China. Biotypes of the different populations were identified and differentiated using aRAPD PCR method with a primer Hi6 . The results showed that 17 whitefly specimens from 11 provinces were B biotype. The datais a good contribution in the distribution and control of Bemisia tabaci B-biotype in China.%收集了国内北京、山东、广东和海南等14个省市23个地区的蔬菜、园林花卉和杂草上的23个烟粉虱Bemisia tabaci(Gennadius)种群,根据报道合成了一个随机引物H16,运用RAPD-PCR技术对所收集的烟粉虱种群进行了生物型鉴定.结果表明在23个烟粉虱种群中,有17个种群同属于危害严重的"B生物型",这些种群主要分布在交通便利的城市或沿海地区,而非B生物型则主要分布在交通不便的山区或内陆.对烟粉虱B生物型的分布与寄主植物和环境条件进行了初步的探讨.