我国白纹伊蚊对三种拟除虫菊酯类杀虫剂抗性检测诊断剂量的建立

摘要

[Aim] To establish the diagnostic doses of deltamethrin,permethrin and lambda-cyhalothrin for detecting resistance in Aedes albopictus in China.[Methods] Insecticide-impregnated papers were made with different concentrations of deltamethrin,permethrin and lambda-cyhalothrin.The susceptibilities of adults of A.albopictus laboratory strain were tested by the standard WHO tube bioassay,and the number of knockdowned individuals at 1 h post treatment and the number of dead individuals at 24 h post treatment were recorded.Log-probit regression,and the LC50 and LC99 values were calculated using Excel 2007 and SPSS20.0 software.The 2-fold LC99 value was used as the diagnostic dose to separate resistant individuals from susceptible individuals.The resistance of a field population of A.albopictus collected from Haikou city to the three pyrethroids was evaluated based on the diagnostic dose determined.[Results] The LC50 values of deltamethrin,lambda-cyhalothrin and permethrin to adult A.albopictus (laboratory strain) were 0.00619％,0.01403％ and 0.05009％,respectively,while the LC99 values were 0.05175％,0.11859％ and 0.53165％,respectively.Thus the corresponding diagnostic doses of the three pyrethroids were 0.1035％,0.2372％ and 1.0633％,respectively.Tested with the diagnostic doses of deltamethrin,permethrin and lambda-cyhalothrin,the mortalities of adults of the field population of Haikou were 22.58％,36.29％ and 40.83％,respectively,indicating that the Haikou population has developed resistance to all the three pyrethroid insecticides.[Conclusion] The diagnostic doses of three pyrethroids established in this study for A.albopictus can be used as the reference for monitoring insecticide resistance of adults of this mosiquito.%[目的]建立中国白纹伊蚊Aedes albopictus成蚊对溴氰菊酯、氯菊酯和高效氯氟氰菊酯杀虫剂抗性检测的诊断剂量.[方法]应用溴氰菊酯、氯菊酯和高效氯氟氰菊酯原药制作不同浓度的药膜滤纸,接触筒法测定白纹伊蚊实验室敏感品系成蚊对3种拟除虫菊酯类杀虫剂的敏感性,记录1h的击倒数和24 h的死亡数.应用Excel 2007和SPSS20.0进行数据统计处理,并制作杀虫剂的毒力回归线,计算各自的LC50和LC99值.以2倍LC99值作为区分抗性和敏感种群的诊断剂量,制作药膜滤纸,接触筒法生物测定海口市白纹伊蚊现场种群成蚊对杀虫剂的抗药性.[结果]溴氰菊酯、高效氯氟氰菊酯和氯菊酯杀虫剂对白纹伊蚊实验室敏感品系成蚊的LC50值分别为0.00619％,0.01403％和0.05009％,LC99值分别为0.05175％,0.11859％,和0.53165％,相对应的诊断剂量分别为0.1035％,0.2372％和1.0633％.应用上述溴氰菊酯、氯菊酯和高效氯氟氰菊酯诊断剂量测定的海口市白纹伊蚊现场种群的死亡率分别为22.58％,36.29％和40.83％,表明该种群对这3种菊酯类杀虫剂均已产生了抗性.[结论]本研究建立的白纹伊蚊对3种拟除虫菊酯类杀虫剂的诊断剂量可作为该蚊成蚊抗药性监测的参考.