补肾健脾益气法对酒精性骨质疏松大鼠骨密度(BMD)、骨矿含量(BMC)影响的研究

摘要

Objective: To observe the influence of the method of tonifying kidney, strengthening spleen and supporting Qi on bone mineral density (BMD) and bone mineral content (BMD) with Alcoholic osteoporosis (AOP) in rats, and to investigate the mechanism of herbs for strengthening kidney and spleen on AOP. Method: 40 adult male SD rats of pathogen - free were randomly divided into four groups on average: model group, western medicine control group, Chinese medicine intervention group and normal control group. The animal model of AOP was induced by irrigation stomach with the Red Start ( Hongxing) Erguotou ( rats in normal control group received saline) , simultaneously, saline, Calcium Carbonate alpha D3, JianGuLing capsule and saline were given to four groups, respectively. Sixteen weeks later, BMD and BMC of thighbone were measured by dual energy X - ray absorptiometry. Results: BMD and BMC in model group were remarkablely decreased compared with normal control group, the difference was significant(P ＜0. 01) , which suggested that rats receiving high concentrations of liguor for 16 weeks really resulted in decrease of BMD and BMC. Furthermore, BMD and BMC in Chinese medicine intervention group(P ＜ 0.01) and western medicine control group (P ＜ 0.05) were remarkablely increased compared with model group. In addition, BMD and BMC in Chinese medicine intervention group were higher than those in Western medicine control group(P ＜ 0.05) , suggesting that Chinese medicine intervention group was superior to western medicine control group. Conclusion: Rats with AOP possess bone metabolism disorder, bone formation decreasing, bone loss reducing and finally induced osteoporosis. The method of tonifying kidney, strengthening spleen and supporting Qi can enhance BMD and BMC, and inhibit loss of bone mineral in rats with AOP, thereby it can effectively improve bone metabolism of rats with AOP.%目的:观察补肾健脾益气法对酒精性骨质疏松(AOP)大鼠骨密度影响,探讨补肾健脾益气中药防治AOP的作用机理.方法:清洁级成年雄性SD大鼠40只,按随机数字表法分为4组,即模型组、西药对照组、中药干预组、正常对照组,每组各10只,用红星二锅头白酒灌胃法造模(正常对照组给予生理盐水灌胃),同时分别给予生理盐水、碳酸钙阿法D3、健骨灵胶囊、生理盐水灌胃给药.16周后取材,用双能X线骨密度仪检测左股骨上端,应用小动物学软件扫描处理,检测出股骨的骨密度(BMD)、骨矿含量(BMC).结果:骨密度(BMD)、骨矿含量(BMC)检测显示:模型组与正常组比较明显降低,有显著性差异(P＜0.01).表明高浓度白酒灌胃大鼠16周后确实存在骨量减少,BMD下降.中药干预组(P＜0.01)、西药对照组(P＜0.05)与模型组相比均有显著升高.中药干预组与西药对照组相比(P＜0.05),说明中药干预组高于西药对照组,有统计学意义.结论:AOP大鼠存在明显的骨代谢紊乱,骨形成减少、骨丢失增加,发生骨质疏松.补肾健脾益气法提高AOP大鼠骨密度和骨矿物质含量,抑制骨矿物质的丢失,有效改善实验性AOP大鼠的骨代谢.