实时荧光定量分析不同锰处理对甘草SQS1基因表达的影响

摘要

目的：探讨不同浓度的锰处理对甘草鲨稀合成酶1（squalene synthetase 1，SQS1或SS1）基因相对表达量的影响。方法：以一年生甘草移栽苗为试验材料，设置5个锰浓度水平，分别为0，1．81，18．1，36．2和54．3mg／L，利用实时荧光定量PCR（Real－time PCR）方法对甘草SQS1基因的Ct值进行测定并计算其相对表达量。结果：随着锰处理浓度的增加甘草SQS1基因的相对表达量呈现先升高后下降的趋势，在18．1mg／L 浓度处理时达到最大值为7．90，分别是对照（0mg／L），1．81mg／L，36．2mg／L 和54.3 mg／L处理的1．75，1．37，1．37，2．33倍，且差异极显著（ P＜0．01）。结论：锰对甘草SQS1基因的表达具有一定地促进作用，适当质量浓度的锰处理能够显著提高甘草SQS1基因的相对表达量。%Objective:To investigate the effect on the relative expression of SQS1 gene by different concentrations of man-ganese ( Mn) of Glycyrrhiza uralensis Fisch..Methods:The transplants of one-year-old G.uralensis were subjected to five concentrations of Mn (0,1.81,18.1,36.2 and 54.3mg/L) culturing in vermiculite.Real-time PCR was used to measure Ct vaule and work out the relative expression quantity of SQS1 gene in different concentrations of Mn of G. uralensis.Results:The expression of SQS1 gene of G.uralensis showed an increase after then with the increase of con-centrations of Mn treatment,it reached the highest 7.90 under 18.1mg/L Mn treatment.It had a significant difference between 18.1mg/L concentration of Mn treatment and 0mg/L,1.81mg/L,36.2mg/L and 54.3mg/L (P<0.01),with 1.75,1.37,1.37,2.33 times respectively.Conclusion: Mn has a certain promoted effect on the expression of SQS1 gene,and the appropriate concentration of Mn treatment can significantly increase the relative expression quantity of SQS1 gene of G.uralensis.