Objective:To establish a culture method of callus induction for Rumex gmelini.Methods:The callus inductions of different explants were compared under different hormone conditions.The content difference of seven kinds of components was also compared in the callus tissue and seedlings by HPLC method.Results:The callus of cotyledons grew early,with large quantity,fast proliferation,bright color,and full vitality,which was beneficial to the subculture and further culture of suspension cells.The cotyledons were induced well in No.2 culture medium (MS + 6-BA 3.0 mg /L +2,4-D 0.1 mg/L),with high induction rate and fast growth speed.The callus is yellow-green and loose,which was good for further culture of cell suspension.The peak areas of most chemical components in the callus were larger.In terms of the seven bioactive components,polydatin and resveratrol in the callus were less than those in the seedling root.The contents of the other five components in the callus,in which a little amount of musizin was especially detected,were more than those in the seedling roots.Conclusion:The research suggests that the cotyledon may be the explants for callus induction,optimal medium is No.2 culture medium (MS + 6-BA 3 mg/1 +2,4-D 0.1 mg/l).The callus contained all the chemical elements in the seedling roots,of which the contents of five components were higher than those in the seedling roots,a little amount of musizin was also found.%目的:建立诱导毛脉酸模愈伤组织的培养方法.方法:比较不同激素配比条件下不同外植体愈伤组织的诱导;采用高效液相色谱法,比较愈伤组织与幼苗中7种指标成分的含量差异.结果:子叶诱导产生的愈伤组织出愈早,量多,增殖快,颜色鲜艳,有生命活力,利于继代培养和进一步进行悬浮细胞的培养;子叶在2号培养基(MS+6-BA 3.0 mg/L+2,4-D0.1 mg/L)中诱导状况良好,愈伤组织诱导率高,并且愈伤组织生长速度快,愈伤组织为黄绿色,比较疏松,有利于愈伤组织进一步的悬浮细胞培养;愈伤组织中多数化学成分的峰面积较大,所测的7种生物活性成分中,愈伤组织中的白藜芦醇苷和白藜芦醇的含量低于幼苗根,其余5种成分含量均高于幼苗根,尤其是在愈伤组织中检测到了少量的酸模素成分.结论:子叶为诱导愈伤组织的外植体,2号培养基(MS +6-BA 3.0 mg/L +2,4-D0.1 mg/L)为诱导愈伤组织的最适培养基;愈伤组织含有幼苗根中的全部化学成分,有5种化学成分的含量高于幼苗根,而且还测得有少量酸模素的存在.
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