茉莉酸甲酯处理雷公藤悬浮细胞的基因差异表达分析

摘要

Methyl Jasmonate (MeJA) with different concentrations was applied to the suspension cultures of Tripterygium wilfordii Hook. f. . The results showed that 50～400 μmol/L MeJA inhibited the accumulation of total alkaloids. cDNA-AFLP (cDNA amplified fragment length polymorphism) analysis found 19 differentially expressed genes between suspension cell culture of T. wilfordii Hook. f. with and without elictor treated. Through Blastn analysis with NCBI protein database, the functions of 7 of them were predicted, which involving in the processes of signal transduction,transcription regulation,energy metabolism and so on. These results will facilitate the improvement alkaloids production using biotechnology methods in the long run.%以不同浓度茉莉酸甲酯(MeJA)为诱导子对雷公藤悬浮细胞进行处理,采用cDNA-AFI,P技术对差异表达基因进行研究.结果表明,茉莉酸甲酯在50～400 μmol/L浓度范围内对雷公藤悬浮细胞总碱的积累呈抑制作用.茉莉酸甲酯处理后,分析筛选出了19个雷公藤悬浮细胞内差异表达的基因.通过与NCBI蛋白质数据库比对,7个片段的功能得以预测,涉及植物细胞的信号转导、转录调控和能量代谢等.这些结果对今后利用生物技术手段提高雷公藤生物碱含量奠定了一定基础.