Mature leaves collected from Vitis davidii ,V .amurensis ,V .heyneana and V .chunganensis were used for chloroplast isolation and cpDNA extraction in this study .The two methods were the column plant chloroplast DNAout and modified high-salt low-pH method ,and the results were compared with each other .(1) Both methods had separated the chloroplast of Chinese wild grapes ,but the modified high-salt low-pH method obtained higher concentration and less impurity of chloroplast than that of column plant chloroplast DNAout .So the modified high-salt low-pH method was more suitable for chloroplast isolation . (2) The value of OD260/OD280 of cpDNA extracted by the column plant chloroplast DNAout was between 1 .28 and 1 .36 ,and the concentration was between 4 .2 ng・μL -1 and 7 .8 ng・μL -1 ,which did not meet the demand of subsequent chloroplast genome sequencing .In contrast ,the value of OD260/OD280 of cpDNA extracted by the modified high-salt low-pH method was between 1 .84 and 1 .90 and the concentration was between 2 514 .4 ng ・ μL -1 and 4 133 .7 ng・ μL -1 ,so the cpDNA extracted in this way was extremely high-quality and pure .As a result ,the cpDNA extracted by the modified high-salt low-pH method meet the demand of subsequent chloroplast genome sequencing .As a conclusion ,the modified high-salt low-pH method isolated intact chloroplast and extract high-quality cpDNA of Chinese wild grapes simply and quickly .And the cpDNA meet the demand of subsequent chloroplast genome sequencing .It was also a critical step to make further research of chloroplast genomes of V itis L .%以中国野生刺葡萄、山葡萄、桑叶葡萄和东南葡萄的成熟叶片为材料，比较柱式植物叶绿体DNAout试剂盒和改良的高盐-低pH法分离叶绿体及提取cpDNA效果。结果显示：（1）2种方法均分离得到了中国野生葡萄的叶绿体，但与柱式植物叶绿体DNAout试剂盒相比，改良的高盐-低pH法得到的叶绿体浓度高、杂质少，更适合中国野生葡萄叶绿体分离。（2）柱式植物叶绿体DNAout试剂盒提取的cpDNA ，其OD260／OD280在1．28～1．36之间，质量浓度为4．2～7．8 ng・μL －1，质量低，不能满足后续测序要求；而改良的高盐-低 pH 法提取的 cpDNA ，其OD260／OD280值在1．84～1．90之间，质量浓度为2514．4～4133．7 ng・μL －1，质量高，纯度好，能满足后续测序要求。研究表明，改良的高盐-低 pH法能简便、快速获得中国野生葡萄完整叶绿体及高质量cpDNA ，并且提取的cpDNA可满足后续测序要求，为葡萄属植物叶绿体基因组的深入研究奠定了基础。
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