Hydrogen peroxide (H2O2) plays an important role in the interaction between plant and pathogen defense response. The objective of this research was owing the interaction system of beet necrotic yellow vein virus (BNYVV) and resistant, susceptible sugarbeet lines to disclose the relationship of accumulation and distribution of H2O2 with sugarbeet rhizomania resistance. The production and accumulation of H2O2 in the two interaction systems were detected and the distribution of H2O2 was observed in subcellular level using the cytochemical technique. The result showed that there was plenty of H2O2 in both interaction systems, and the production of H2O2was evidently higher in the resistant lines than with in the susceptible lines. In addition, distribution and the location of H2O2 were similar in the two lines, where we observed mainly in the tonoplast and the plasma membrane of host root, vein cells as well as the intercellular space of some host vein cells. But there were obvious differences in the content of H2O2 accumulated in the two interaction lines, the deposition of H2O2 in the susceptible line was significantly lower than that in the resistant line, showing it may have a close relationship with resistance of sugarbeet against rhizomania, H2O2 showed the involved in the defense responses to virus infection in sugar beets.%以甜菜抗、感丛根病品种与甜菜坏死黄脉病毒互作体系为研究对象,检测不同体系间H2O2的产生和积累,并利用电镜细胞化学标记技术在亚细胞水平上对H2O2的空间分布定位,探讨H2O2积累和分布与甜菜抗丛根病性的关系.结果表明,甜菜抗、感丛根病体系均出现大量H2O2,其中抗病体系的H2O2产生量明显高于感病体系.H2O2在抗病和感病体系中的分布位置基本相似,多分布在块根、叶脉细胞的液泡膜和质膜上,叶脉细胞间隙也有H2O2的分布,但不同体系间H2O2含量有着明显差异,感病体系H2O2沉积量明显弱于抗病体系.研究结果说明,H2O2产生量和分布与甜菜抗丛根病性有密切联系,H2O2参与了甜菜对病毒侵染的防御反应.
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