北京地区连作草莓根际土壤中真滑刃线虫的鉴定

摘要

Aphelenchus sp. ,a dominant nematode species in Beijing continuous-cropping strawberry soil, was identi-fied in present study by morphological and molecular methods. The results showed that its morphology was extremely similar with Aphelenchus avenae Bastian. Besides, the main morphological measurements of Aphelenchus sp. and Aph-elenchus avenae Bastian were consistent. On the basis of morphological identification results, combining with ITS rD-NA regional sequence amplification results with GenBank registered sequence alignment analysis, it was determined that Aphelenchus sp. was a new strain of Aphelenchus avenae. The identification of Aphelenchus sp. laid a solid foun-dation for the further study of its biology and ecology characteristics. Since Aphelenchus sp. existed in large numbers in the rhizosphere soil of continuous-cropping strawberry, it's needed to carry out further research to know whether it have adverse effect on the growth of strawberry or not.%对北京地区连作草莓土壤中占种群优势地位的真滑刃线虫Aphelenchus sp.,采用传统形态学和分子生物学相结合的手段进行了鉴定.结果表明,文中分离得到的真滑刃线虫,虫体形态与燕麦真滑刃Aphelen-chus avenae Bastian极为相似,且主要形态学测量值基本相符.在形态学鉴定结果的基础上,结合ITS-rDNA区域序列扩增结果与GenBank已注册的序列比对分析,确定该线虫为燕麦真滑刃线虫.该线虫的分类鉴定为其生物学及生态学特性的深入研究奠定了基础,但由于该线虫在连作草莓的根际土壤中大量存在,其是否对草莓的生长有不利影响值得进一步探讨.