5种大量元素对青钱柳愈伤组织生长及黄酮类化合物积累的影响

摘要

The effects of five macronutrients in MS agar medium on the growth and flavonoid accumulation of Cyclocarya paliurus Callus were studied. The results showed that decreasing the concentrations of NH4NO3 and KNO3 to 1/2C in MS medium favored the growth and flavonoids accumulation of callus. In contrast, 3/2C KH2PO4 was the best concentration of medium, in which the dry weight of callus and flavonoids concentration were 0.52 g/bottle and 4.67% , respectively. The effect of MgSO4 concentration showed that medium with 3/2C MgSO4 was suitable for callus growth, while for flavonoid accumulation 1/2C MgSO4 was better. CaCl2 had no significant effect on callus growth. However, increasing Ca2+ concentration improved flavonoids accumulation in the medium.%以MS为基本培养基,研究不同浓度的5种大量元素对青钱柳愈伤组织生长及黄酮类化合物积累的影响.结果表明:MS培养基中NH4 NO3、KN03浓度分别降至1/2C时,有利于愈伤组织生长和黄酮积累；当KH2P04浓度为3/2C时,愈伤组织生长量与黄酮含量均达到最大值,分别为0.53g/瓶和4.67%；MgSO4浓度在3/2C时,愈伤组织生长良好,而1/2C有利于黄酮积累；CaCl2在一定范围内不会影响愈伤组织生长,适当提高Ca2+浓度有利于黄酮积累.