水稻白叶枯病菌北方菌株遗传多样性rep - PCR分析

摘要

为了揭示水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae,Xoo)北方菌株的遗传结构组成,利用rep - PCR 技术对103个从辽宁、吉林、河北、山东稻区采集的Xoo菌株、9个标准小种(R1 -R9)代表菌株和13个水稻细菌性条斑病菌(X.oryzae pv.oryzicola,Xoc)菌株进行了遗传多样性分析.结果表明,ERIC -PCR和BOX-PCR分析均能较好地揭示出测试菌株的遗传多态性,分别获得了84种和90种分子谱型；在相似系数为70%时,ERIC -PCR将所有测试菌株聚类为17个簇群,其中1个为优势簇群；BOX - PCR将测试菌株聚类为10个簇群,其中2个为优势簇群.在相似系数为50%时,Xoo与Xoc测试菌株分别聚类为2个不同的簇群.%To reveal the genetic structure of Xanthomonas oryzae pv. Oryzae (Xoo) strains in the north rice -growing regions of China, 103 strains isolated from the diseased rice leaves collected from Liaoning, Ji-lin, Hebei and Shandong Provinces, 9 representative strains of pathotypes/races (Rl - R9) of Xoo and 13 strains of X. Oryzae pv. Oryzicola ( Xoc) were analyzed using rep-PCR technique. Dendrograms were generated from the combination data sets of primers ERIC and BOX by using UPGMA analysis. The results indicated that extensive genetic diversity existed within the population of Xoo strains tested, they showed 84 and 90 hap-lotypes respectively. All tested strains could be divided into 17 clusters at level of 70% similarity using primer ERIC, and there was one predominant cluster, but 10 clusters using primer BOX and two predominant clusters. All tested strains of Xoo and Xoc could be divided into two different clusters respectively at a level of 50% similarity.