用分子标记对部分高代育种材料抗稻瘟基因Pi9(t)的检测

摘要

用分子标记PB8对课题组新育成的部分高代育种材料进行抗稻瘟病基因Pi9(t)的检测,检测到27份材料中含Pi9(t)基因、11份材料含Pi9(t)纯合基因.对含Pi9(t)纯合基因的材料进行了四川主要稻瘟病生理小种的接种鉴定,其抗性频率速78.13％ ～87.50％,表明Pi9(t)基因是四川稻区稻瘟病的主效抗病基因,对四川主要稻瘟病生理小种表现出较高的抗性.%This paper applied molecular marker PB8 to identify rice blast resistant gene Pi9(t) in some high -generation breeding materials. It was found that 27 materials contained Pi9(t) gene, and 11 materials contained homozygous Pi9{t) gene. The materials containing homozygous Pi9(t) gene were inoculated by Sichuan provincial major physiological races of rice blast, and their resistance frequency reached 78.13% ~87.50% , indicating that Pii(l) gene was the major resistant gene to rice blast in Sichuan rice are-a, and it showed strong resistance to the major rice blast physiological races in Sichuan.'