蓝莓夏普蓝组培苗繁殖技术研究

摘要

以蓝莓“夏普蓝”品种为试验材料研究了组培苗繁殖技术。在培养基中添加不同浓度ZT、蔗糖，分析了其对组培苗丛生芽诱导增殖的影响，以及不同IBA浓度对组培苗生根的影响。结果表明：改良WPM＋ZT 1．25 mg/L培养条件下，“夏普蓝”组培苗丛生芽分化效果最佳。在培养基中附加蔗糖是蓝莓增殖培养所必需的，蔗糖浓度过高或过低，都可能对蓝莓增殖产生不良影响，以蔗糖40 g/L时的增殖效果最好；生长素IBA对“夏普蓝”的生根诱导有促进作用，IBA 0．6 mg/L对诱导蓝莓组培苗生根最有效，生根率可达88％，生根速度较快，粗细均匀，根系壮。%The propagation techniques of tissue -culture seedlings of blueberry variety “Xiapulan” were studied .The influ-ences of various media containing different concentrations of ZT and sucrose on the induced propagation of cluster buds of the tissue-culture seedlings , as well as the effects of different concentrations of IBA on the rooting of the tissue -culture seedlings were ana-lyzed.The results showed that the differentiation of cluster buds of “Xiapulan” tissue-culture seedlings was the best on the im-proved WPM+ZT 1.25 mg/L medium.It was necessary for the propagation of blueberry to add sucrose into the medium , but over-high or too low concentration of sucrose could cause adverse effect on the propagation of blueberry , and 40 g/L sucrose had the best effect.The auxin IBA had promotive action on the rooting of tissue -culture seedlings of “Xiapulan”, and 0.6 mg/L IBA had the best rooting effect:the rooting rate reached 88%, the rooting speed was relatively high , and the roots were strong and uniform in thickness .