N-苯基-2-萘胺对青菜种子萌发及幼苗生理参数变化的影响

摘要

The seeds of Chinese cabbage were soaked by the various concentrations of N-phenyl-2-naphthyl-amine. The effects of N-phenyl-2-naphthylamine on the seed germination,the growth,the chlorophyll content and the change of antioxidant enzymes in Chinese cabbage were studied in this paper. The results showed that the germination energy and the germination percentage increased with the increase of N-phenyl-2-naphthylamine concentration, whereas they did not have significant difference compared to the control. The change trends of the germination index and the vigor index were the same with the germination energy and the germination percentage. The germination index and the vigor index increased under the lower N-phenyl-2-naphthylamine concentration, but there were no difference compared to the control. When the N-phenyl-2-naphthylamine concentration was less than 1.00 mg/L,the shoot height increased with the increase of N-phenyl-2-naphthylamine concentration. Especially, in the 0.50 mg/L treatment, the shoot height was biggest and had significant difference compared to the control. The trend of the root length change liked as the fresh weight. The root length and fresh weight indicated the trend of increase-decrease with the increase of N-phenyl-2-naphthylamine concentration. Under the lower N-phenyl-2-naphthylamine concentration, the chlorophyll content and antioxidant enzymes activities markedly increased with the increase of N-phenyl-2-naphthylamine concentration. However, the chlorophyll content and antioxidant enzymes activities markedly decreased compared with the control in the higher N-phenyl-2-naphthylamine concentration.%以青菜为材料,研究了不同浓度N-苯基-2-萘胺对青菜种子进行浸种后对种子发芽、生长、叶绿素含量及抗氧化酶活性的影响.结果表明,N-苯基-2-萘胺浸种可以提高种子的发芽势和发芽率,但与对照相比差异不显著；发芽指数和活力指数的变化趋势与发芽势和发芽率的变化趋势相似,在低浓度N-苯基-2萘胺处理下,发芽指数和活力指数增加,但与对照相比差异不显著；当N-苯基-2萘胺浓度小于1 mg/L时,随着N-苯基-2萘胺浓度的增加,青菜的株高不同程度增加,在0.50 mg/L处理下,株高增加最多,显著大于对照；根长和鲜质量的变化趋势相似,随着N-苯基-2-萘胺使用量的增加呈现先增加而后降低.在低浓度N-苯基-2-萘胺处理下,青菜体内的叶绿素含量和抗氧化酶活性与对照相比显著增加,当使用高浓度N-苯基-2-萘胺处理后,叶绿素含量与抗氧化酶活性和对照相比显著降低.