小麦返白系相关基因的克隆与表达

摘要

In order to study the molecular mechanism of the " albino-after green" in wheat ( Triticum aestivum L. ) albinism line, we have designed the PCR primer with the LSC of Chloroplasts genome sequence in Chinese spring wheat. We have amplified the related gene segment in chinese spring wheat, albinism line and Aibian 1 and get the specific gene fragment WFC01 in albinism line. The total RNA has been extracted from leaves in Chinese spring wheat, Aibian 1 and albinism line. WFC01 was the probe for northern blot. The results show that the expression of gene WFC01 has been inhibition along with the leaves albinism in albinism line compared with albinism line and Chinese spring wheat. The gene WFC01 expression was back to normal with the leaves get green.%为研究小麦返白系阶段性“白化-复绿”的分子机制,以中国春小麦叶绿体基因组序列的LSC区为参考序列,分段设计引物,PCR扩增中国春、矮变1号、返白系的相应基因片段,在返白系中获得特异基因片段WFC01.提取中国春、矮变1号幼嫩叶片和返白系白化及复绿叶片总RNA,毛细管法转膜,WFC01为探针进行Northern杂交.结果表明,与对照矮变1号和中国春相比,返白系随着叶片的白化,WFC01基因片段的表达受到明显抑制,几乎无表达,随着叶片的复绿,WFC01基因片段的表达恢复正常,与对照表达量一致,表明该基因的表达与返白系的阶段性白化、复绿有关.