捕食线虫性真菌的分离鉴定及氨基酸对其捕食器诱导效应

摘要

为了解新疆阿勒泰地区的捕食线虫性真菌分布情况,采用玉米粉琼脂培养基( CMA)培养分离阿勒泰地区土壤中的捕食线虫性真菌,通过对纯化后菌株的形态学观察和分子生物学分析,鉴定出5株少孢节丛孢属真菌和1株圆锥节丛孢菌。另外用质量浓度分别为0.5,0.05,0.005 g/L的L-苯丙氨酸、L-色氨酸、L-蛋氨酸和L-缬氨酸对已分离的圆锥节丛孢菌AS3株进行捕食器生成的诱导反应,发现各组氨基酸对捕食器的产生均有诱导效应,但质量浓度为0.05 g/L的L-苯丙氨酸诱导产生的捕食器总数最多。为利用捕食线虫性真菌进行生物防控奠定了理论基础。%Nematode-trapping fungi were isolated with corn meal agar( CMA) from soils samples of Altay region in Xinjiang Province. 6 strains were successfully isolated and five strains identified as Arthrobotrys oligospora and the other one is Arthrobotrys conoides through morphological characteristics and molecular biology analysis. In addition, four amino acids (L-phenylalanine,L-tryptophan,L-methionine and L-valine) in different concentrations (0. 5, 0. 05,0. 005 g/L) were used as revulsive to induce the nematode-trapping organs forming of AS3 strain. The results showed that all the amino acids had effects on the traps forming,but the L-phenylalanine in concentration of 0. 05 g/L induced maximum number of trapping devices. This study laid a good foundation for biological prevention and control by nematode-trapping fungi.