NF-YB型转录因子在介导植物抵御非生物逆境中发挥着重要作用。为揭示小麦该家族成员 TaNF-YB2；1在干旱和盐分逆境下的表达特征及超表达 TaNF-YB2；1对植株抵御上述逆境能力的影响。利用半定量 RT-PCR 技术，研究TaNF-YB2；1的表达特征，采用农杆菌介导的遗传转化技术，建立了超表达TaNF-YB2；1的转基因烟草植株。结果表明，TaNF-YB2；1 cDNA全长序列为958 bp，编码163个氨基酸残基。在24 h PEG 模拟干旱和盐分处理条件下，TaNF-YB2；1的转录本数量明显上调。正常培养下，与野生型植株相比，转基因植株的生长和干物质积累量未发生改变；但在干旱和盐分处理下，转基因株系植株的生长较野生型植株明显改善，表现为植株个体体积增大，单株干物质积累量增多。因此，TaNF-YB2；1的表达受到干旱和盐分逆境的明显诱导，通过对干旱和盐分逆境应答，该基因在介导植株抵御上述环境逆境中发挥着重要功能。%NF-YB type transcription factor family plays critical roles in mediating plant tolerance to diverse abi-otic stresses. The purpose of this study was to understand the expression patterns of TaNF-YB2;1,a NF-YB tran-scription factor gene in wheat,and determine the function of this gene in regulating plant tolerance to aforemen-tioned stresses. The expression patterns of TaNF-YB2;1 were determined based on semiquantitative RT-PCR and transgenic tobacco plants overexpressing TaNF-YB2;1 were generated by adopting the Agrobacteriun-mediated ge-netic transformation approach. The results indicated that TaNF-YB2;1 had a cDNA full length of 958 bp,encoded a 163 amino acid-polypeptide. Under the conditions of drought and salt stress,the transcripts of TaNF-YB2;1 were drastically upregualted in comparison those under the condition of normal growth,suggested that it responds to a-bove stressors. Under normal growth,the growth features and dry mass of the plants overexpressing TaNF-YB2;1 were similar to wild type. However,the plants overexpressing TaNF-YB2;1 exhibited significantly improved growth features and dry mass compared with the wild type plants under treatments of drought and salt. Therefore,the ex-pression of TaNF-YB2;1 was dramatically induced by stresses of drought and high salinity. TaNF-YB2;1 acts as a critical regulator in mediating plant tolerance to drought and salt through its response to above stressors.
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