重组人BDNF对Aβ25-35致原代培养海马神经元损伤的保护作用

摘要

Objective To observe the protective effect of brain-derived neurotrophic factor(BDNF) on A β 25-35- induced primarily cultured hippocampal neuron injury. Methods Hippocampal cells taken from new-born Wistar rats were incubated in vitro, into which different concentrations of BDNF and aggregated A β 25-35 were added to act on the cultured hippocampal neurons. Effect of BDNF on the survival rate of hippocampal neurons and apoptosis of hippocampal cells induced by A β 25-35 was observed by MTT and Hoechst 33342 staining, respectively. Results The damage of hippocampal cells was less severe in BDNF treatment group than in A β 25-35 model group, and the hippocampal cells grew well under microscope. MTT showed that the survival rate of hippocampal cells increased with the increased concentration of BDNF. Hoechst 33342 staining showed that the apoptosis rate of hippocampal cells decreased with the increased concentration of BDNF, which was lower in BDNF treatment group than in A β 25-35 model group(P<0.01). Conclusion BDNF can protect the cultured hippocampal neurons against injury induced by A β 25-35 due to its neurotrophic activity.%目的 观察人脑源性神经营养因子(BDNF)对Aβ 25-35致原代培养海马神经元损伤的保护作用.方法 取新生wistar大鼠海马细胞进行体外培养.分别加入不同浓度的BDNF及聚集态A β 25-35作用于培养的海马神经元,采用MTT法观察BDNF对A β 25-35致海马神经元存活率影响及Hoechst 33342染色观察BDNF对A β 25-35致海马细胞凋亡的影响.结果 显微镜下观察BDNF处理组比A β 25-35模型组细胞损伤小,细胞生长良好.MTT结果显示随着BDNF浓度增高,细胞存活率逐渐增高,在0.6nmol/L时最明显,与A β 25-35模型组比较,细胞存活率显著增高(P＜0.01).Hoechst 33342染色显示随着BDNF浓度增大凋亡率减少,与A β 25-35组比较凋亡率均显著减少(P＜0.01).结论 BDNF对培养海马神经元有神经营养活性,对Aβ致培养海马神经元的损伤有保护作用.