Acceptor reactions and mechanism of Leuconostoc mesenteroides B-512FM dextransucrase.

摘要

Although Hanes-Woolf plot showed that methyl ;A model, based on the known mechanisms of dextran synthesis and acceptor reactions, is proposed to explain the inhibition of dextran synthesis, and the increase in the acceptor products at high sucrose concentrations. According to the model, sucrose binds to a third, low-affinity binding site, allosterically changing the conformation of the active site so that dextran cannot be formed but acceptor products can be formed.;Sugar mixtures containing isomaltodextrins were produced from sucrose using dextransucrase.