The present invention provides a Leuconostoc mesenteroides CJNU 0041 strain having high activity of β-glucosidase, and the broccoli fermented product fermented with the above-described Leukonostoc mesenteroides CJNU 0041 strain is an active ingredient. It provides a description of the composition for preventing, treating or improving colitis. The composition according to the present invention includes broccoli fermented product prepared by fermenting broccoli with a leukonostock mesenteroides strain (CJNU 0041) having high activity of β-glucosidase as an active ingredient, which is a causative agent causing colitis. Can effectively inhibit the growth of Clostridium difficile strains can be utilized for the purpose of preventing, treating or improving colitis.
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