In vitro studies with mismatched double-stranded ribonucleic acid.

摘要

ouble stranded ribonucleic acid (ds-RNA) is a known interferon inducer. A mismatched form, p I;In asymptomatic HIV-infected subjects who received Ampligen (40-570 mg/m;Mixing human or rabbit serum with Ampligen or p I:C resulted in masking of the antiviral activity in cell culture. Heating the serum, before or after mixing, resulted in amelioration of that effect. Fifty percent protection was observed with 10 ;There was a sudden drop in white cell count in patients after administration of Ampligen, followed by rebound two hours later To examine this phenomenon, we used RK-2 cell monolayers and U-937 cells in suspension. Both Ampligen (p ;The antiviral activity was assessed. Rabbit kidney cell and chick-embryo cell cultures in 96 well plates were treated with the ds-RNA before challenge with vesicular stomatitis virus (VSV) or encephalomyocarditis virus (EMC). Reduction in cytopathology and virus yield was demonstrated using dye binding methods. Both Ampligen and p I:C were active at low concentrations. Ampligen, 44 ng/ml, provided 50% protection against VSV effect in RK-2 cells. More than 98% virus yield reduction was achieved in RK-2 with Ampligen concentration 0.32