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Fecal bacteria survival in cow manure and Escherichia coli release and transport through porous media.

机译:粪便细菌在牛粪和大肠杆菌中的存活通过多孔介质释放和运输。

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The overall goal of this dissertation was to describe fecal bacteria transport through the environment.; The survival of indicator bacteria (fecal coliforms, Escherichia coli and fecal streptococci) in dairy cow manure over a range of environmental temperatures (4, 27, and 41°C) and manure moisture contents (30%, 55%, and 83%) was studied. After an initial 3–10 days growth period, the coliforms showed significant reductions in responses to temperature but not moisture content after 3 weeks or less. The streptococci showed no significant reductions after three months. Manure still could contaminate water resources after 103 days.; E. coli adsorption was investigated by comparing responses between silt loam and sand. Up to 108 of E. coli cells were sorbed per gram of the silt loam soil whereas only 104 cells attached to the same mass of sand. Both Freundlich (R2 = 0.89) and Langmuir (R2 = 0.93) isotherms described observed adsorption data well for soil but a linear isotherm did not. Results showed that E. coli adsorption to both soil and sand particles was reversible.; The effect of fine (1 mm) vs. coarse (>1 mm and 2 mm) heterogeneous sand media on breakthrough of E. coli was compared. A second-order kinetic adsorption model coupled with the convection-dispersion equation was used to describe E. coli transport through sand columns packed with those two media. Model parameters were also estimated. A total of 97% E. coli cells were recovered from the effluent of the coarse sand column whereas only 52% from that of the fine sand column. The model fitted the coarse sand better (model efficiency = 91%) than that of the fine sand (61%).; The impact of antecedent soil water content (5.3%, 72% and 99% saturation) on overall retention of E. coli in silt loam soil columns was investigated. Most tested columns (93%) had a cell removal efficiency of over 99.999%. The lowest removal efficiency (75%) and greatest E. coli cell concentration (2.7 × 107 cfu/mL) were found in the effluent from saturated columns.; Information obtained from this study can be used to assess pathogen sources when developing TMDLs.
机译:本文的总体目标是描述粪便细菌在环境中的运输。在环境温度(4、27和41°C)和粪便水分含量(30%,30%)的条件下,指示菌(粪大肠菌群,大肠杆菌和粪便链球菌)在奶牛粪肥中的存活率。 55%和83%)进行了研究。在最初的3-10天的生长期后,大肠菌对温度的反应显着降低,但3周或更短的时间后水分含量没有下降。三个月后,链球菌无明显减少。 103天后,肥料仍会污染水资源。 <斜体> E。通过比较粉壤土和砂土之间的响应,研究了大肠埃希菌的吸附。最多10 8 E。每克淤泥质壤土会吸附大肠杆菌,而只有10 4 细胞附着在相同质量的沙子上。 Freundlich(R 2 = 0.89)和Langmuir(R 2 = 0.93)等温线都描述了很好的土壤吸附数据,但线性等温线却没有。结果显示<斜体> E。大肠埃希菌对土壤和沙粒的吸附是可逆的。细颗粒(<1 mm)与粗糙颗粒(> 1 mm和<2 mm)的非均质砂介质对 E穿透的影响。比较了大肠杆菌。使用二阶动力学吸附模型和对流扩散方程来描述E。大肠杆菌通过装有这两种培养基的沙柱运输。还估算了模型参数。从粗砂塔的流出物中总共回收了97%的大肠杆菌细胞,而从细砂塔的流出物中仅回收了52%。该模型比粗砂(61%)更适合粗砂(模型效率= 91%)。前期土壤含水量(5.3%,72%和99%的饱和度)对<斜体> E总体保留的影响。研究了粉壤土土壤柱中的大肠埃希菌。大多数测试柱(93%)的细胞去除效率均超过99.999%。饱和柱流出物中的去除效率最低(75%),最大大肠杆菌细胞浓度(2.7×10 7 cfu / mL)。从本研究获得的信息可用于在开发TMDL时评估病原体来源。

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