Novel near-infrared cyanine dyes for fluorescence imaging in biological systems.

摘要

A series of meso-amino substituted heptamethine cyanine dyes with indolenine, benz[e]indolenine and benz[c,d]indolenine heterocyclic moieties were synthesized and photophysical properties were measured in ethanol. High quantum yields are observed for primary amine derivatives. Fluorescence quantum yields are greater for dyes with indolenine end groups than with benz[e]indolenine end groups. 5-HT6 and 5-HT7 receptors belong to the G-protein coupled receptor superfamily that are abundant in the CNS and are implicated in multiple disorders of the brain and other regulatory processes. Novel pyrimidine-based monomeric and dimeric small molecules were synthesized based on previous results. Initial in vitro studies (Table 1) show that extended flexible chain derivatives have higher selectivity towards the receptors compared to other compounds. Hydrophobic linker derivatives of the dimers are more selective to 5-HT 7 than hydrophilic linker derivatives.;INDEX WORDS: Near-infrared, Heptamethine, Heterocycle, Indolenine, Benz[ e]indolenine, Benz[c,d]indolenine, Fluorescence, Spectroscopy, Fluorophores, Photosensitizer, Benzothiazole, Quantum yield, Nonlinear, Photophysical, Serotonin, Pharmacophore.