Chronic and acute regulation of intestinal triglyceride metabolism genes by dietary fat.

摘要

Excess dietary fat is associated with obesity and metabolic syndrome. The small intestine efficiently absorbs a range of dietary triglyceride, suggesting intestinal adaptation to various triglyceride intakes. In the current study, we analyze intestinal mRNA levels of proteins involved in intestinal triglyceride metabolism in lean and diet induced obese mice. Obese mice show increased intestinal adipose differentiation related protein, peroxisome proliferator-activated receptor alpha, peroxisome proliferator-activated receptor gamma, acyl-CoA oxidase, diacylglycerol acyltransferase 2, apolipoprotein A-IV, fatty acid transporter and liver fatty acid binding protein mRNA levels. We also analyzed mRNA levels of proteins involved in intestinal triglyceride metabolism in lean and diet induced obese mice in response to a high fat challenge. Lean mice have increased tail interacting protein of 47 kDa, adipose differentiation related protein, adipose triglyceride lipase, comparative gene identification 58, diacylglycerol acyltransferase 2, microsomal triglyceride transfer protein and fatty acid transporter mRNA levels post bolus. Conversely, diet induced obese mice do not have increased mRNA levels post bolus and lipolytic enzyme mRNA levels are decreased. Lipid droplet associated protein levels also dynamically increase and later decrease post bolus in lean mice. Increased lipid droplet associated protein levels are blunted in diet induced obese mice. Overall, the small intestinal mucosa adapts to both diet induced obesity and a high fat challenge in lean and diet induced obese mice.