This study investigated the presence of axonal injury within cervical facet joint capsules (FJC) exposed to a high-rate (100 mm/s) tensile stretch. The left C5-C6 FJCs of five anaesthetized goats were subjected to a series of tensile tests in 4 mm increments until rupture (the intact right FJCs served as controls). The FJCs were harvested, fixed in 4% buffered paraformaldehyde, embedded in paraffin, and serially sectioned. FJC sections were immunolabeled for neurofilament light chain (NF-L) and beta-amyloid precursor protein (beta-APP). A significantly higher frequency of coupled beta-APP/NF-L immunoreactive sections was found in stretched (23.8%) compared to unstretched FJCs (6.3%, p = 0.02). This finding suggests that high-rate tensile stretch is a mechanism for axonal injury in cervical FJCs, and furthers the understanding of axonal injury in the whiplash pain mechanism. The use of the dual immunolabeling, presents a new method for identifying axonal injury in skeletal tissue.
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机译：这项研究调查了暴露于高速（100 mm / s）拉伸拉伸的颈椎小关节囊（FJC）中是否存在轴突损伤。对五只麻醉山羊的左侧C5-C6 FJC进行一系列的拉伸测试，以4 mm的增量进行直至破裂（完整的右侧FJC作为对照）。收获FJC，固定在4％的低聚甲醛中，包埋在石蜡中，然后连续切片。对FJC切片进行神经丝轻链（NF-L）和β-淀粉样蛋白前体蛋白（β-APP）免疫标记。与未拉伸的FJCs（6.3％，p = 0.02）相比，拉伸（23.8％）的β-APP/ NF-L偶联免疫反应性切片的频率明显更高。这一发现表明，高速率拉伸是宫颈FJC中轴突损伤的一种机制，并进一步促进了对鞭打痛机制中轴突损伤的了解。双重免疫标记的使用，提供了一种识别骨骼组织中轴突损伤的新方法。
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