hGCP3 and psoriasin: Is their interaction biologically significant in human breast cancer?

摘要

An increasing body of evidence suggests that centrosomal dysfunction is a contributory factor, rather than side effect of early cancer progression. Our lab has identified psoriasin (S100A7) as an important player in promoting early breast cancer progression. To understand psoriasin's intracellular function we sought to identify putative psoriasin-interacting proteins with a yeast-2-hybrid (Y2H) assay. Therein we identified two centrosomal associated proteins as candidate psoriasin-interacting proteins. This has led us to hypothesize that psoriasin may promote cellular properties associated with cancer progression, in part, by influencing centrosomal function.; The aim of the current study was to determine whether one of these candidates, gamma-tubulin ring complex protein-3 (hGCP3) physically interacts with psoriasin and whether this interaction is biologically relevant in breast cancer cells.; It is concluded that although an interaction between hGCP3 and psoriasin can occur in the Y2H system, it does not appear that this interaction is conserved in mammalian cells. Moreover, the absence of changes in centrosomal function when psoriasin is overexpressed supports this conclusion and suggests that a psoriasin-hGCP3 interaction is not biologically significant in human breast cancer. (Abstract shortened by UMI.)