A real-time snapshot hyperspectral endoscope and miniature endomicroscopy objectives for a two field-of-view (Bi-FOV) endoscope.

摘要

This thesis focuses on the development of two novel imaging technologies which will serve as the basis for the future development of a two field-of-view or "Bi-FOV" endoscope capable of imaging at both microscopic and macroscopic regimes simultaneously. The goal of the Bi-FOV is to provide clinicians with a better tool for pre- and early cancer detection. The first technology developed makes it possible to obtain low cost, high performance, miniature optical systems for use in the microscopic portion of the Bi-FOV endoscope. The second technology developed for the Bi-FOV is a real-time snapshot hyperspectral endoscope called the IMS endoscope. The IMS endoscope is based on an image mapping technique which is capable of achieving high temporal and spatial resolution, excellent optical throughput, and low costs. The parallel, high throughput nature of this technique enables the device to operate at frame rates of 5.2 fps while collecting a 3D (x, y, gamma ) datacube of 350 x 350 x 48. We have successfully imaged tissue in vivo resolving tissue vasculature and oxy-hemoglobin which are important early cancer biomarkers.